European Commission > EU Science Hub > EURL GMFF
Legal Notice   Privacy statement   English (EN)
Welcome to the GMOMETHODS application

GMOMETHODS provides information on EU reference methods for GMO Analysis.

The assays are DNA-based detection methods that have been validated in a collaborative study according to ISO 5725 and/or the International Union of Pure and Applied Chemistry (IUPAC) requirements. In alternative, the assays have been verified by the EURL GMFF for EU legal purposes. Data are retrieved from peer-reviewed journals and final reports of collaborative studies.

The application assists control laboratories in selecting appropriate methods for GMO analysis, supplies core data on the experimental protocol and information on methods performance, ring-trial design, plasmid standards, reference materials and links to published articles or validation reports.

Perform your search by inserting a key word or by selecting a GMO unique identifier.

Only one hit for query 'ac:ACS-GM006-4'
ID   QT-EVE-GM-007; SV 0; linear; genomic DNA; STS; SYN; 75 BP.
AC   ACS-GM006-4;
DT   07-APR-2008
DT   15-MAR-2012
DE   Quantitative PCR method for detection of soybean event A5547-127
DE   (Charles Delobel et al., 2009).
KW   event_specific.
OS   Glycine max (soybean) - event A5547-127 (ACS-GM006-4)
RN   [1]
RP   1-75
RA   Charles Delobel C., Bogni A., Mazzara M., Van Den Eede G.;
RT   "Event-specific Method for the Quantification of Soybean Line A5547-127 Using Real-time PCR - Validation Report and Protocol";
RL   Online Publication (2009).
RX   DOI=10.2788/60083
RN   [2]
RP   1-75
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QT-EVE-GM-007.pdf
FH   Key             Location/Qualifiers
FT   STS             1..75
FT                   /standard_name="PCR 75 bp amplicon"
FT                   /note="event-specific RT-PCR"
FT                   /target="5' integration border region (IBR) between the insert of soybean event A5547-127 and the soybean host genome"
FT   primer_bind     1..22
FT                   /standard_name="Primer forward: SHA003"
FT                   /note="GCTATTTGGTGGCATTTTTCCA"
FT                   /target="5'-host genome"
FT   primer_bind     28..53
FT                   /standard_name="RT-PCR probe: TM058"
FT   primer_bind     complement(55..75)
FT                   /standard_name="Primer reverse: SHA004"
FT                   /note="CACTGCGGCCAACTTACTTCT"
FT                   /target="insert"
SQ   Sequence 75 BP; 14 A; 15 C; 18 G; 22 T; 6 other;
     gctatttggt ggcatttttc cannnnnccg caatgtcata ccgtcatcgt tgtnagaagt        60
     aagttggccg cagtg                                                         75