Only one hit for query 'ac%3AACS-GM006-4'
ID QL-ELE-00-024; SV 1; linear; genomic DNA; STD; PLN; 87 BP.
XX
AC ;
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DT 20-FEB-1985
DT 22-DEC-2016
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DE Qualitative PCR method for detection of tE9 terminator (Debode et al., 2016).
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KW element_specific.
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RN [1]
RP 1-87
RA Debode F., Huber I., Macarthur R., Rischitor P.E., Mazzara M., Herau V., Sebah D., Dobnik D., Broeders S., Roosens N.H., Busch U., Berben G., Morisset D., Zel J.;
RT "Inter-laboratory studies for the validation of two singleplex (tE9 and pea lectin) and one duplex (pat/bar) real-time PCR methods for GMO detection";
RL Food Control 73:452-461 (2017).
RX DOI=10.1016/j.foodcont.2016.08.037.
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RN [2]
RP 1-87
RT "PCR reactions set up and amplification conditions";
RL Online Publication (2016).
RX PCR=QL-ELE-00-024.pdf
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FH Key Location/Qualifiers
FH
FT STS 1..87
FT /standard_name="PCR 87 bp amplicon"
FT /note="element-specific PCR"
FT /target="Terminator of the pea ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) E9 gene"
FT primer_bind 1..23
FT /standard_name="Primer forward: T-E9-R"
FT /note="TTTTTATTCGGTTTTCGCTATCG"
FT /target="T-E9"
FT primer_bind complement(25..60)
FT /standard_name="RT-PCR probe: T-E9-P"
FT /note="FAM-TCATTAACTCTTCTCCATCCATTTCCATTTCACAGT-TAMRA"
FT primer_bind complement(62..87)
FT /standard_name="Primer reverse: T-E9-F"
FT /note="TGAGAATGAACAAAAGGACCATATCA"
FT /target="T-E9"
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SQ Sequence 87 BP; 23 A; 10 C; 20 G; 34 T; 0 other;
tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa gagttaatga 60
atgatatggt ccttttgttc attctca 87
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