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ID   QL-CON-00-008; SV 0; linear; genomic DNA; STS; SYN; 88 BP.
AC   ;
DT   04-AUG-2009
DT   05-OCT-2010
DE   Qualitative PCR method for detection of the junction between the chloroplast transit peptide 2 and the CP4 epsps gene
DE   (Grohmann et al., 2009).
KW   construct_specific.
RN   [1]
RP   1-88
RA   Grohmann L., Brunen-Nieweler C., Nemeth A., Waiblinger H.U.;
RT   "Collaborative trial validation studies of real-time PCR-based GMO
RT   screening methods for detection of the bar gene and the ctp2-cp4epsps
RT   construct";
RL   J. Agric. Food Chem. 57:8913-8920 (2009).
RX   PUBMED; 19807158.
RX   DOI=10.1021/jf901598r
RN   [2]
RP   1-88
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QL-CON-00-008.pdf
FH   Key             Location/Qualifiers
FT   STS             1..88
FT                   /standard_name="PCR 88 bp amplicon"
FT                   /note="construct-specific RT-PCR"
FT                   /target="Junction region between the chloroplast transit peptide 2 (CTP2) sequence from the Arabidopsis thaliana epsps gene and the CP4 epsps gene from Agrobacterium tumefasciens (CP4 EPSPS)"
FT   primer_bind     1..22
FT                   /standard_name="Primer forward: GT73-TmF"
FT                   /note="GGGATGACGTTAATTGGCTCTG"
FT                   /target="CTP2"
FT   primer_bind     23..69
FT                   /standard_name="RT-PCR probe: GT73-TmP"
FT   primer_bind     complement(70..88)
FT                   /standard_name="Primer reverse: GT73-TmR"
FT                   /note="GGCTGCTTGCACCGTGAAG"
FT                   /target="CP4-EPSPS"
SQ   Sequence 88 BP; 8 A; 10 C; 13 G; 10 T; 47 other;
     gggatgacgt taattggctc tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnnnnnnnnc ttcacggtgc aagcagcc                                           88