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ID   QT-EVE-ZM-023; SV 0; linear; genomic DNA; STS; SYN; 82 BP.
XX
AC   SYN-EV176-9;
XX
DT   30-NOV-2011
DT   18-JAN-2021
XX
DE   Quantitative PCR method for detection of maize event Bt176 (verified by the EU-RL GMFF in the context of Commission Decision 2007/304/EC)
XX
KW   event_specific, EU-RL_GMFF_in-house_verified.
XX
OS   Zea mays (maize) - event Bt176 (SYN-EV176-9)
XX
RN   [1]
RP   1-82
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "In-house Validation of an Event-specific Method for the Quantification of Maize Bt176 Using Real-time PCR - Validation Report and Protocol";
RL   Online Publication (2011).
RX   EURL_GMFF=Bt176_CRLVL1804_VR.pdf
RX   EURL_GMFF=Bt176_CRLVL1804_validated_Method.pdf
XX
RN   [2]
RP   1-82
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2011).
RX   PCR=QT-EVE-ZM-023.pdf
XX
DR   GMOMETHODS; QT-TAX-ZM-014;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..82
FT                   /standard_name="PCR 82 bp amplicon"
FT                   /note="event-specific RT-PCR"
FT                   /target="3'integration border region (IBR) between the insert of maize event Bt176 and the maize host genome";
FT   primer_bind     1..18
FT                   /standard_name="Primer forward: Bt176 F"
FT                   /note="GGCCGTGAACGAGCTGTT"
FT                   /target="insert"
FT   primer_bind     26..47
FT                   /standard_name="RT-PCR probe: Bt176 Probe"
FT                   /note="FAM-AGCAACCAGATCGGCCGACACC-TAMRA"
FT   primer_bind     complement(58..82)
FT                   /standard_name="Primer reverse: Bt176 R"
FT                   /note="GGGAAGAAGCCTACATGTTTTCTAA"
FT                   /target="3'-host genome"
XX
SQ   Sequence 82 BP; 21 A; 26 C; 18 G; 17 T; 0 other;
     ggccgtgaac gagctgttnn nnnnnagcaa ccagatcggc cgacaccnnn nnnnnnntta        60
     gaaaacatgt aggcttcttc cc                                                 82
//