ID   QT-EVE-ZM-001; SV 0; linear; genomic DNA; STS; SYN; 85 BP.
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AC   VCO-01981-5;
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DT   11-JAN-2013
DT   14-JUL-2016
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DE   Quantitative PCR method for detection of maize event VCO-01981-5 (EURL GMFF, 2016).
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KW   event_specific.
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OS   Zea mays (maize) - event VCO-01981-5 (VCO-01981-5)
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RN   [1]
RP   1-85
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Maize VCO-01981-5 Using Real-time PCR - Validation Report and Validated Method";
RL   Online Publication (2016).
RX   EURL_GMFF=EURL-VL-07-12-VR.pdf
RX   EURL_GMFF=EURL-VL-07-12-VP.pdf
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RN   [2]
RP   1-85
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2016).
RX   PCR=QT-EVE-ZM-001.pdf
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DR   GMOMETHODS; QT-TAX-ZM-004;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..85
FT                   /standard_name="PCR 85 bp amplicon"
FT                   /note="Event-specific RT-PCR;
FT                   /target="5' integration border region (IBR) between the insert of maize event VCO-01981-5 and the maize host genome";
FT   primer_bind     1..22
FT                   /standard_name="Primer forward: VCO-01981-5 primer F"
FT                   /note="CCACTGAACGTCACCAAGAAGA"
FT                   /target="5'-host genome"
FT   primer_bind     24..43
FT                   /standard_name="RT-PCR probe: VCO-01981-5 probe"
FT                   /note="FAM-CAGTACTCAAACACTGATAG-MGB"
FT   primer_bind     complement(66..85)
FT                   /standard_name="Primer reverse: VCO-01981-5 primer R"
FT                   /note="GCCGCTACTCGAGGGATTTA"
FT                   /target="insert"
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SQ   Sequence 85 BP; 31 A; 20 C; 15 G; 19 T; 0 other;
     ccactgaacg tcaccaagaa gancagtact caaacactga tagnnnnnnn nnnnnnnnnn        60
     nnnnntaaat ccctcgagta gcggc                                              85
//