ID   QL-CON-00-015; SV 0; linear; genomic DNA; STS; SYN; 136 BP.
XX
AC   ;
XX
DT   16-JUL-2013
DT   18-OCT-2021
XX
DE   Qualitative PCR method for detection of the junction between the CaMV35S promoter and the bar gene (verified by the EURL GMFF in the context of Commission Decision 2006/578/EC).
XX
KW   construct_specific, EURL_GMFF_in-house_verified.
XX
OS   Oryza sativa (rice).
XX
RN   [1]
RP   1-136
RA   Bayer CropScience;
RT   "Grain testing method for detection of rice GM event LLRICE601 using RT-PCR protocols PGS0505 and PGS0476";
RL   Online Publication (2006).
RX   CRL-DOC=P35S-bar sqRT-PCR - PGS0494-476 310806.pdf
XX
RN   [2]
RP   1-136
RA   US Department of Agriculture;
RT   "Verification of the Bayer CropScience method for the detection of LL601 in rice using real-time PCR";
RL   Online Publication (2006).
RX   CRL-DOC=35SBarRiceVerficationReportrev.pdf
XX
RN   [3]
RP   1-136
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Report on the Verification of a Construct-specific Detection Method for
RT   Identification of Rice GM-Events containing P35S::BAR using a Real-time
RT   PCR Assay";
RL   Online Publication (2006).
RX   CRL-DOC=Verification Report 35S-BAR CRL.pdf
XX
RN   [4]
RP   1-136
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2006).
RX   PCR=QL-CON-00-015.pdf
XX
DR   GMOMETHODS; QL-TAX-OS-002;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..136
FT                   /standard_name="PCR 136 bp amplicon"
FT                   /note="Construct-specific RT-PCR"
FT                   /target="Junction region between the Cauliflower Mosaic Virus 35S promoter (CaMV P-35S) and the phosphinothricin N-acetyl transferase (bar) gene from Streptomyces hygroscopicus"
FT   misc_feature    1..136
FT                   /note="The fragment size generated by the CaMV P-35S-bar PCR depends on the assembly of the two sequence elements and is varying in the different GM plant events"
FT   primer_bind     1..21
FT                   /standard_name="Primer forward: MDB498"
FT                   /note="TATCCTTCGCAAGACCCTTCC"
FT                   /target="CaMV P-35S"
FT   primer_bind     22..46
FT                   /standard_name="RT-PCR probe: TM099"
FT                   /note="FAM-TCTATATAAGGAAGTTCATTTCATT-MGBNFQ"
FT   primer_bind     complement(115..136)
FT                   /standard_name="Primer reverse: DPA143"
FT                   /note="ATGTCGGCCGGGCGTCGTTCTG"
FT                   /target="bar gene"
XX
SQ   Sequence 136 BP; 37 A; 41 C; 21 G; 37 T; 0 other;
     tatccttcgc aagacccttc ctctatataa ggaagttcat ttcattnnnn nnnnnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncagaac       120
     gacgcccggc cgacat                                                       136
//