ID QL-ELE-00-014; SV 0; linear; genomic DNA; STS; SYN; 60 BP.
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AC ;
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DT 04-AUG-2009
DT 05-OCT-2010
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DE Qualitative PCR method for detection of phosphinothricin N-acetyltransferase (bar) gene
DE (Grohmann et al., 2009).
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KW element_specific.
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RN [1]
RP 1-60
RA Grohmann L., Brunen-Nieweler C., Nemeth A., Waiblinger H.U.;
RT "Collaborative trial validation studies of real-time PCR-based GMO
RT screening methods for detection of the bar gene and the ctp2-cp4epsps
RT construct";
RL J. Agric. Food Chem. 57:8913-8920 (2009).
RX PUBMED; 19807158.
RX DOI=10.1021/jf901598r
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RN [2]
RP 1-60
RT "PCR reactions set up and amplification conditions";
RL Online Publication (2010).
RX PCR=QL-ELE-00-014.pdf
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FH Key Location/Qualifiers
FH
FT STS 1..60
FT /standard_name="PCR 60 bp amplicon"
FT /note="element-specific RT-PCR"
FT /target="phosphinothricin N-acetyltransferase (bar) gene from bacterium Streptomyces hygroscopicus"
FT primer_bind 1..19
FT /standard_name="Primer forward: RapB-F1"
FT /note="ACAAGCACGGTCAACTTCC"
FT /target="bar"
FT primer_bind 20..42
FT /standard_name="RT-PCR probe: RapB-S1"
FT /note="FAM-TACCGAGCCGCAGGAACC-TAMRA"
FT primer_bind complement(43..60)
FT /standard_name="Primer reverse: RapB-R1"
FT /note="GAGGTCGTCCGTCCACTC"
FT /target="bar"
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SQ Sequence 60 BP; 10 A; 12 C; 10 G; 5 T; 23 other;
acaagcacgg tcaacttccn nnnnnnnnnn nnnnnnnnnn nngagtggac ggacgacctc 60
//