ID QL-TAX-CMV-001; SV 1; circular; genomic DNA; STD; VRL; 68 BP. XX AC ; XX DT 14-NOV-2023 DT 14-NOV-2023 XX DE Qualitative PCR method for detection of CaMV ORF V (ISO/TS 21569-7, 2022). XX KW taxon_specific, validated_independently. XX OS Cauliflower mosaic virus XX RN [1] RP 1-68 RT "Horizontal methods for molecular biomarker analysis - Methods of RT analysis for the detection of genetically modified organisms and derived RT products - Part 7: Real-time PCR based methods for the detection of CaMV RT and Agrobacterium Ti-plasmid derived DNA sequences"; RL ISO/TS 21569-7 (2022). RX ISO=84434 XX RN [2] RP 1-68 RT "PCR reactions set up and amplification conditions"; RL Online Publication (2023). RX PCR=QL-TAX-CMV-001.pdf XX FH Key Location/Qualifiers FH FT STS 1..68 FT /standard_name="PCR 68 bp amplicon" FT /note="Taxon-specific RT-PCR" FT /target="Cauliflower mosaic virus (CaMV) open reading frame V (ORF V)" FT primer_bind 1..20 FT /standard_name="Primer forward: CaMV-ORFV-fd2" FT /note="ATYAAGCCCAGYAAAAGCCC" FT /target="CaMV ORF V" FT primer_bind 24..48 FT /standard_name="RT-PCR probe: CaMV-ORFV-P2" FT /note="FAM-CATGGCACCAGCCTTCTTGGTCAAC-BHQ1" FT primer_bind complement(49..68) FT /standard_name="Primer reverse: CaMV-ORFV-rd2" FT /note="CTYCGCTTCTCGGCTTCRTT" FT /target="CaMV ORF V" XX SQ Sequence 68 BP; 23 A; 19 C; 15 G; 11 T; 0 other; atyaagccca gyaaaagccc nnncatggca ccagccttct tggtcaacaa ygaagccgag 60 aagcgrag 68 //