ID   QT-EVE-GM-011; SV 0; linear; genomic DNA; STD; PLN; 88 BP.
XX
AC   BPS-CV127-9;
XX
DT   20-OCT-2011
DT   29-NOV-2012
XX
DE   Quantitative PCR method for detection of soybean event CV127
DE   (Savini et al., 2011)
XX
KW   event_specific.
XX
OS   Glycine max (soybean) - event CV127 (BPS-CV127-9)
XX
RN   [1]
RP   1-88
RA   Savini C. et al.,;
RT   "Event-specific Method for the Quantification of Soybean CV127 Using Real-time PCR - Validation Report and Protocol";
RL   Online Publication (2011).
RX   EURL_GMFF=BPS-CV-127-9_validated Method.pdf
RX   EURL_GMFF=BPS-CV127-9_validationReport.pdf
XX
RN   [2]
RP   1-88
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2011).
RX   PCR=QT-EVE-GM-011.pdf
XX
DR   GMOMETHODS; QT-TAX-GM-002;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..88
FT                   /standard_name="PCR 88 bp amplicon"
FT                   /note="Event-specific RT-PCR"
FT                   /target="3' integration border region (IBR) between the insert of soybean event CV127 and the soybean host genome";
FT   primer_bind     1..28
FT                   /standard_name="Primer forward: SE-127-f4"
FT                   /note="AACAGAAGTTTCCGTTGAGCTTTAAGAC"
FT                   /target="insert"
FT   primer_bind     30..52
FT                   /standard_name="RT-PCR probe: SE-127-p3"
FT                   /note="FAM-TTTGGGGAAGCTGTCCCATGCCC-TAMRA"
FT   primer_bind     complement(66..88)
FT                   /standard_name="Primer reverse: SE-127-r2"
FT                   /note="CATTCGTAGCTCGGATCGTGTAC"
FT                   /target="3'-host genome"
XX
SQ   Sequence 88 BP; 27 A; 20 C; 22 G; 19 T; 0 other;
     aacagaagtt tccgttgagc tttaagacnt ttggggaagc tgtcccatgc ccnnnnnnnn        60
     nnnnngtaca cgatccgagc tacgaatg                                           88
//