ID QT-EVE-BN-013; SV 0; linear; genomic DNA; STD; SYN; 123 BP. XX AC BPS-BFLFK-2; XX DT 01-SEP-2021 DT 19-AUG-2022 XX DE Quantitative PCR method for detection of oilseed rape event LBFLFK locus2 (EURL GMFF, 2022). XX KW event_specific. XX OS Brassica napus (rape) - event LBFLFK (BPS-BFLFK-2) XX RN [1] RP 1-123 RA European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission; RT "Event-specific Method for the Quantification of Oilseed Rape LBFLFK Locus1 and Locus2 Using Real-time PCR - Validation Report"; RL Online Publication (2022). RX EURL_GMFF=EURL-VL-02-19-VR.pdf RX EURL_GMFF=EURL-VL-02-19-VM.pdf XX RN [2] RP 1-123 RT "PCR reactions set up and amplification conditions"; RL Online Publication (2022). RX PCR=QT-EVE-BN-013.pdf XX DR GMOMETHODS; QT-TAX-BN-001; XX FH Key Location/Qualifiers FH FT STS 1..123 FT /note="Event-specific RT-PCR" FT /target="5' integration border region (IBR) between the FT insert of oilseed rape event LBFLFK Locus2 and the FT oilseed rape host genome" FT primer_bind 1..28 FT /standard_name="Primer forward: rightFST_ev1_loc2-f4" FT /note="GATTGGTAATATGTAAATAACGGGATCC" FT /target="5'-host genome" FT primer_bind 50..74 FT /standard_name="RT-PCR probe: rightFST_ev1_loc2-s1" FT /note="FAM-CATCACACCAAAAGTTAGGCCCGAA-BHQ1" FT primer_bind complement(104..123) FT /standard_name="Primer reverse: rightFST_ev1_loc2-r1" FT /note="GCGAATTTGGCCTGTAGACC" FT /target="insert" XX SQ Sequence 123 BP; 44 A; 24 C; 24 G; 31 T; 0 other; gattggtaat atgtaaataa cgggatccnn nnnnnnnnnn nnnnnnnnnc atcacaccaa 60 aagttaggcc cgaannnnnn nnnnnnnnnn nnnnnnnnnn nnnggtctac aggccaaatt 120 cgc 123 //