ID   QL-ELE-00-017; SV 1; linear; genomic DNA; STD; VRT; 75 BP.
XX
AC   ;
XX
DT   03-NOV-1982
DT   29-FEB-2016
XX
DE   Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Barbau-Piednoir et al., 2014).
XX
KW   element_specific.
XX
RN   [1]
RP   1-75
RA   Barbau-Piednoir E., Stragier P., Roosens N., Mazzara M., Van den Eede G.,
RA   Van den Bulcke M.;
RT   "Inter-laboratory Testing of GMO Detection by Combinatory SYBR Green PCR
RT   Screening(CoSYPS)";
RL   Food Analitical Methods 0:0-0 (201402
RX   DOI=10.1007/s12161-014-9837-3
XX
RN   [2]
RP   1-75
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2014).
RX   PCR=QL-ELE-00-017.pdf
XX
DR   GMOMETHODS; QL-TAX-BN-003;
DR   GMOMETHODS; QL-TAX-GM-001;
DR   GMOMETHODS; QL-TAX-ZM-002;
DR   GMOMETHODS; QL-PLN-00-006;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..75
FT                   /standard_name="PCR 75 bp amplicon"
FT                   /note="element-specific PCR"
FT                   /target="Cauliflower Mosaic Virus 35S promoter (CaMV P-35S)"
FT   primer_bind     1..23
FT                   /standard_name="Primer forward: 35S_N3Fwd"
FT                   /note="AAAGCAAGTGGATTGATGTGATA"
FT                   /target="CaMV P-35S"
FT   primer_bind     complement(56..75)
FT                   /standard_name="Primer reverse: 35S_N3Rev"
FT                   /note="GGGTCTTGCGAAGGATAGTG"
FT                   /target="CaMV P-35S"
XX
SQ   Sequence 75 BP; 23 A; 20 C; 16 G; 16 T; 0 other;
     aaagcaagtg gattgatgtg atannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncacta        60
     tccttcgcaa gaccc                                                         75
//