ID   QT-EVE-GM-002; SV 0; linear; genomic DNA; STS; SYN; 87 BP.
XX
AC   MON-87769-7;
XX
DT   30-OCT-2009
DT   07-FEB-2012
XX
DE   Quantitative PCR method for detection of soybean event MON87769 (Savini et al., 2012)
XX
KW   event_specific.
XX
OS   Glycine max (soybean) - event MON87769 (MON-87769-7)
XX
RN   [1]
RP   1-87
RA   Savini C.,;
RT   "Event-specific Method for the Quantification of Soybean MON87769 Using Real-time PCR - Validation Report and Protocol";
RL   Online Publication (2012).
RX   EURL_GMFF=2012-01-27_CRLVL0709 MON87769_VP.pdf
RX   EURL_GMFF=2012-01-27_CRLVL0709 MON87769_VR.pdf
XX
RN   [2]
RP   1-87
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2012).
RX   PCR=QT-EVE-GM-002.pdf
XX
DR   GMOMETHODS; QT-TAX-GM-002;
XX
FH   Key             Location/Qualifiers
FH                   
FT   STS             1..87
FT                   /standard_name="PCR 87 bp amplicon"
FT                   /note="Event-specific RT-PCR"
FT                   /target="3'integration border region (IBR) between the insert of soybean event MON87769 and the soybean host genome";
FT   primer_bind     1..27
FT                   /standard_name="Primer forward: MON 87769 primer 1"
FT                   /note="CATACTCATTGCTGATCCATGTAGATT"
FT                   /target="insert";
FT   primer_bind     29..56
FT                   /standard_name="RT-PCR probe: MON 87769 probe"
FT                   /note="FAM-CCCGGACATGAAGCCATTTACAATTGAC-TAMRA"
FT   primer_bind     complement(66..87)
FT                   /standard_name="Primer reverse: MON 87769 primer 2"
FT                   /note="GCAAGTTGCTCGTGAAGTTTTG"
FT                   /target="3'-host genome";
XX
SQ   Sequence 87 BP; 27 A; 24 C; 12 G; 24 T; 0 other;
     catactcatt gctgatccat gtagattncc cggacatgaa gccatttaca attgacnnnn        60
     nnnnncaaaa cttcacgagc aacttgc                                            87
//