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Welcome to the GMOMETHODS application

GMOMETHODS provides information on EU reference methods for GMO Analysis.

The assays are DNA-based detection methods that have been validated in a collaborative study according to ISO 5725 and/or the International Union of Pure and Applied Chemistry (IUPAC) requirements. In alternative, the assays have been verified by the EURL GMFF for EU legal purposes. Data are retrieved from peer-reviewed journals and final reports of collaborative studies.

The application assists control laboratories in selecting appropriate methods for GMO analysis, supplies core data on the experimental protocol and information on methods performance, ring-trial design, plasmid standards, reference materials and links to published articles or validation reports.

Perform your search by inserting a key word or by selecting a GMO unique identifier.

Only one hit for query 'ac:MON-87419-8'
ID   QT-EVE-ZM-029; SV 0; linear; genomic DNA; STD; PLN; 97 BP.
AC   MON-87419-8;
DT   13-SEP-2019
DT   13-SEP-2019
DE   Quantitative PCR method for detection of maize event MON87419 (EURL GMFF, 2019).
KW   event_specific.
OS   Zea mays (maize) - event MON87419 (MON-87419-8).
RN   [1]
RP   1-97
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Maize MON 87419 Using Real-time PCR";   
RL   Online Publication (2019).
RX   EURL_GMFF=EURL-VL-02_17_VR_MON87419.pdf
RX   EURL_GMFF=EURL-VL-02_17_VM_MON87419.pdf
RN   [2]
RP   1-97
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2019).
RX   PCR=QT-EVE-ZM-029.pdf
FH   Key             Location/Qualifiers
FT   STS             1..97
FT                   /standard_name="PCR 97 bp amplicon"
FT                   /note="Event-specific RT-PCR";
FT                   /target="5' integration border region 
FT 				(IBR) between the insert of maize event MON87419 and the maize host genome";
FT   primer_bind     1..19
FT                   /standard_name="Primer forward: 87419 QF"
FT                   /note="CGGTCGCTGCCAGGTATTG"
FT                   /target="5'-host genome"
FT   primer_bind     21..46
FT                   /standard_name="RT-PCR probe: 87419 QP"
FT   primer_bind     complement(75..97)
FT                   /standard_name="Primer reverse: 87419 QR"
FT                   /note="CAGACCTCAATTGCGAGCTTTCT"
FT                   /target="insert"
SQ   Sequence 97 BP; 27 A; 22 C; 25 G; 23 T; 0 other;
     cggtcgctgc caggtattgn tgtgcgccag tcagcatcat cacaccnnnn nnnnnnnnnn        60
     nnnnnnnnnn nnnnagaaag ctcgcaattg aggtctg                                 97