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Welcome to the GMOMETHODS application

GMOMETHODS provides information on EU reference methods for GMO Analysis.

The assays are DNA-based detection methods that have been validated in a collaborative study according to ISO 5725 and/or the International Union of Pure and Applied Chemistry (IUPAC) requirements. In alternative, the assays have been verified by the EURL GMFF for EU legal purposes. Data are retrieved from peer-reviewed journals and final reports of collaborative studies.

The application assists control laboratories in selecting appropriate methods for GMO analysis, supplies core data on the experimental protocol and information on methods performance, ring-trial design, plasmid standards, reference materials and links to published articles or validation reports.

Perform your search by inserting a key word or by selecting a GMO unique identifier.

Only one hit for query 'ac:SYN-IR604-5'
ID   QT-EVE-ZM-013; SV 0; linear; genomic DNA; STS; SYN; 76 BP.
AC   SYN-IR604-5;
DT   29-MAY-2006
DT   20-OCT-2010
DE   Quantitative PCR method for detection of maize event MIR604
DE   (Mazzara et al., 2007).
KW   event_specific.
OS   Zea mays (maize) - event MIR604 (SYN-IR604-5)
RN   [1]
RP   1-76
RA   Mazzara M.,  Munaro B., Foti N., Savini C., Van Den Eede G.;
RT   "Event-specific Method for the Quantification of Maize Line MIR604 Using Real-time PCR - Validation Report and Protocol - Maize Seeds Sampling and DNA Extraction";
RL   Online Publication (2007).
RX   DOI=10.2788/30596
RN   [2]
RP   1-76
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QT-EVE-ZM-013.pdf
FH   Key             Location/Qualifiers
FT   STS             1..76
FT                   /standard_name="PCR 76 bp amplicon"
FT                   /note="event-specific RT-PCR"
FT                   /target="5' integration border region (IBR) between the insert of maize event MIR 604 and the maize host genome"
FT   primer_bind     1..18
FT                   /standard_name="Primer forward: MIR604 primer F"
FT                   /note="GCGCACGCAATTCAACAG"
FT                   /target="5'-host genome"
FT   primer_bind     20..45
FT                   /standard_name="RT-PCR probe: MIR604 Probe"
FT   primer_bind     complement(51..76)
FT                   /standard_name="Primer reverse: MIR604 primer R"
FT                   /target="insert"
SQ   Sequence 76 BP; 24 A; 15 C; 19 G; 12 T; 6 other;
     gcgcacgcaa ttcaacagna ggcgggaaac gacaatctga tcatgnnnnn agaattaagg        60
     gagtcacgtt atgacc                                                        76