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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)


Perform your search by keyword, select a GMO unique identifier or click a link in the section below.


Only one hit for query 'ac:DAS-01507-1'
ID   QT-EVE-ZM-010; SV 0; linear; genomic DNA; STS; SYN; 58 BP.
XX
AC   DAS-01507-1;
XX
DT   17-JAN-2007
DT   07-OCT-2010
XX
DE   Quantitative PCR method for detection of maize event TC1507
DE   (Mazzara et al., 2005).
XX
KW   event_specific.
XX
OS   Zea mays (maize) - event TC1507 (DAS-01507-1)
XX
RN   [1]
RP   1-58
RA   Mazzara M., Foti N., Price S., Paoletti C., Van Den Eede G.;
RT   "Event-Specific Method for the Quantitation of Maize Line TC1507 Using Real-Time PCR - Validation Report and Protocol - Sampling and DNA Extraction of Maize TC15O7";
RL   Online Publication (2005).
RX   BSHOP=LBNA21828
XX
RN   [2]
RP   1-58
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QT-EVE-ZM-010.pdf
XX
DR   GMOMETHODS; QT-TAX-ZM-002;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..58
FT                   /standard_name="PCR 58 bp amplicon"
FT                   /note="event-specific RT-PCR"
FT                   /target="integration border region (IBR) between the insert of maize event TC1507 and the maize host genome";
FT   primer_bind     1..19
FT                   /standard_name="Primer forward: MaiY-F1"
FT                   /note="TAGTCTTCGGCCAGAATGG"
FT                   /target="not specified"
FT   primer_bind     22..42
FT                   /standard_name="RT-PCR probe: MaiY-S1"
FT                   /note="FAM-TAACTCAAGGCCCTCACTCCG-TAMRA"
FT   primer_bind     complement(41..58)
FT                   /standard_name="Primer reverse: Maiy-R3"
FT                   /note="CTTTGCCAAGATCAAGCG"
FT                   /target="not specified"
XX
SQ   Sequence 58 BP; 13 A; 18 C; 13 G; 14 T; 0 other;
     tagtcttcgg ccagaatggn ntaactcaag gccctcactc cgcttgatct tggcaaag          58
//