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EU Database of Reference Methods for GMO Analysis
Search for Select by GMO Unique Identifier:
Only one hit for query 'ac:BCS-GH002-5'
Entry information
Entry name QT-EVE-GH-006;       See in JRC GMO-Matrix       See in JRC GMO-Amplicons
GMO Unique Identifier BCS-GH002-5
Description Quantitative PCR method for detection of cotton event GHB614 (Savini et al., 2008).
Keywords event_specific.
From Gossypium hirsutum (upland cotton) - event GHB614 (BCS-GH002-5)
1 Savini C., Bogni A., Mazzara M., Van Den Eede G.; "Event-specific Method for the Quantification of Cotton Line GHB614 Using Real-time PCR - Validation Report and Protocol" Online Publication (2008)
DOI 10.2788/43697
Reference Position 1-120
2 "PCR reactions set up and amplification conditions" Online Publication (2010)
PCR QT-EVE-GH-006.pdf
Reference Position 1-120
Key Location Qualifier Value
STS 1..120 standard_namePCR 120 bp amplicon
noteevent-specific RT-PCR
target3' integration border region (IBR) between the insert of cotton event GHB614 and the cotton host genome
primer_b 1..24 standard_namePrimer forward: SHA007
primer_b 31..60 standard_nameRT-PCR probe: TM072
noteThe probe sequence provided by the applicant is missing a nucleotide at position 51 of the amplicon according to patent sequence accession no. CS492094
primer_b complement(100..120) standard_namePrimer reverse: SHA008
target3'-host genome
Sequence information
Length: 120 BP, A Count: 20, C Count: 19, T Count: 22, G Count: 14
caaatacact tggaacgact tcgtnnnnnn ctccatggcg atcgctacgt ntctagaatt        60
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ttaaaagctt gcatgcctgc       120