Perform your search by keyword, select a GMO unique identifier or click a link in the section below.


ID   QT-CON-00-003; SV 0; linear; genomic DNA; STS; SYN; 74 BP.
XX
AC   MON-04032-6;
XX
DT   29-MAY-2009
DT   06-OCT-2016
XX
DE   Quantitative PCR method for detection of the junction between the CaMV35S promoter and the CTP sequence (ISO/FDIS 21570:2005).
XX
KW   construct_specific.
XX
OS   Glycine max (soybean) - event GTS-40-3-2 (MON-04032-6)
XX
RN   [1]
RP   1-74
RT   "Foodstuffs - Methods of analysis for the detection of genetically
RT   modified organisms and derived products - Quantitative nucleic acid based
RT   methods";
RL   ISO 21570:1-103 (2005).
RX   ISO=34615
XX
RN   [2]
RP   1-74
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QT-CON-00-003.pdf
XX
DR   GMOMETHODS; QT-TAX-GM-002;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..74
FT                   /standard_name="PCR 74 bp amplicon"
FT                   /note="Construct-specific RT-PCR"
FT                   /target="Junction region between the Cauliflower Mosaic Virus 35S promoter (CaMV P-35S) and the chloroplast transit peptide (CTP) sequence from Petunia hybrida epsps gene"
FT   primer_bind     1..21
FT                   /standard_name="Primer forward: RR1-F"
FT                   /note="CATTTGGAGAGGACACGCTGA"
FT                   /target="CaMV P-35S"
FT   primer_bind     22..45
FT                   /standard_name="RT-PCR probe: RR1"
FT                   /note="FAM-CAAGCTGACTCTAGCAGATCTTTC-TAMRA"
FT   primer_bind     complement(52..74)
FT                   /standard_name="Primer reverse: RR1-R"
FT                   /note="GAGCCATGTTGTTAATTTGTGCC"
FT                   /target="CTP4"
XX
SQ   Sequence 74 BP; 25 A; 17 C; 16 G; 16 T; 0 other;
     catttggaga ggacacgctg acaagctgac tctagcagat ctttcnnnnn nggcacaaat        60
     taacaacatg gctc                                                          74
//