ID QL-EVE-DC-006; SV 0; linear; genomic DNA; STD; SYN; 338 BP. XX AC FLO-40685-2; XX DT 25-OCT-2013 DT 08-DEC-2017 XX DE Qualitative PCR method for detection of carnation event 123.8.8 (verified by the EURL GMFF, 2016). XX KW event_specific, EURL_GMFF_in-house_verified. XX OS Dianthus caryophyllus (carnation) - event 123.8.8 (FLO-40685-2) XX RN [1] RP 1-338 RA European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission; RT "Report on the Single-laboratory Validation of a PCR-based Detection Method for Identification of GM-line FLO-40685-2 Carnation - JRC Validated Methods, Reference Methods and Measurement Reports"; RL Online Publication (2016). RX EURL_2001=EURL-EV-02-13-VR.pdf XX RN [2] RP 1-338 RT "PCR reactions set up and amplification conditions"; RL Online Publication (2017). RX PCR=QL-EVE-DC-006.pdf XX DR GMOMETHODS; QL-TAX-DC-001; XX CC NOTE: This GMO was formerly referred to with the FLO-40685-1 Unique CC identifiers (UI) which OECD corrected (JAN 2015). XX FH Key Location/Qualifiers FH FT STS 1..338 FT /standard_name="PCR 338 bp amplicon" FT /note="Event-specific PCR for the second insertion site FT of GMO event 123.8.8 carnation"; FT /target="3' integration border region (IBR) between the insert of carnation event 123.8.8 and the carnation host genome"; FT primer_bind 1..22 FT /standard_name="Primer forward: RBF1a" FT /note="TGGTGGACCCTTGAGGAAACTG" FT /target="insert" FT primer_bind complement(317..338) FT /standard_name="Primer reverse: 123.8.8-2.1R" FT /note="ATACAATGGCTGACTGATGTAG" FT /target="3'-host genome" XX SQ Sequence 338 BP; 90 A; 60 C; 82 G; 106 T; 0 other; tggtggaccc ttgaggaaac tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 60 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 300 nnnnnnnnnn nnnnnnctac atcagtcagc cattgtat 338 //