Perform your search by keyword, select a GMO unique identifier or click a link in the section below.


ID   QL-ELE-00-022; SV 1; linear; other DNA; STD; UNA; 69 BP.
XX
AC   ;
XX
DT   25-JAN-2010
DT   29-FEB-2016
XX
DE   Qualitative PCR method for detection of phosphinothricin N-acetyltransferase (bar) gene (Barbau-Piednoir et al., 2014).
XX
KW   element_specific.
XX
RN   [1]
RP   1-69
RA   Barbau-Piednoir E., Stragier P., Roosens N., Mazzara M., Van den Eede G.,
RA   Van den Bulcke M.;
RT   "Inter-laboratory Testing of GMO Detection by Combinatory SYBR Green PCR
RT   Screening(CoSYPS)";
RL   Food Analitical Methods 0:0-0 (2014).
RX   DOI=10.1007/s12161-014-9837-3
XX
RN   [2]
RP   1-69
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2014).
RX   PCR=QL-ELE-00-022.pdf
XX
DR   GMOMETHODS; QL-TAX-BN-003;
DR   GMOMETHODS; QL-TAX-GM-001;
DR   GMOMETHODS; QL-TAX-ZM-002;
DR   GMOMETHODS; QL-PLN-00-006;
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    1..69
FT                   /note="Sybricon007 insert containing Streptomyces
FT                   hygroscopicus partial bar gene"
FT   STS             1..69
FT                   /standard_name="PCR 69 bp amplicon"
FT                   /note="element-specific PCR"
FT                   /target="phosphinothricin N-acetyltransferase (bar) gene from bacterium Streptomyces hygroscopicus"
FT   primer_bind     1..23
FT                   /standard_name="Primer forward: Pat-Bar Fwd"
FT                   /note="CGTCAACCACTACATCGAGACAA"
FT                   /target="bar"
FT   primer_bind     complement(51..69)
FT                   /standard_name="Primer reverse: Pat-Bar Rev"
FT                   /note="GTCCACTCCTGCGGTTCCT"
FT                   /target="bar"
XX
SQ   Sequence 69 BP; 20 A; 23 C; 18 G; 8 T; 0 other;
     cgtcaaccac tacatcgaga caannnnnnn nnnnnnnnnn nnnnnnnnnn aggaaccgca        60
     ggagtggac                                                                69
//