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ID   QL-ELE-00-008; SV 1; linear; genomic DNA; STD; BCT; 94 BP.
XX
AC   ;
XX
DT   26-DEC-2012
DT   13-JUN-2017
XX
DE   Qualitative PCR  method for detection of nopaline synthase promoter (P-nos).
XX
KW   element_specific.
XX
RN   [1]
RP   1-94
RT   "Detection of the P-nos sequence to screen for components of genetically modified organisms (GMOs) in foodstuffs using real-time PCR";
RL   BVL L 00.00-141:2013-01 (2013).
RX   BVL=bvl-l-0000-141/179718826
XX
RN   [2]
RP   1-94
RT   "Horizontal methods for molecular biomarker analysis -- Methods of analysis for the detection of genetically modified organisms and derived products -- Part 4: Real-time PCR based screening methods for the detection of the P-nos and P-nos-nptII DNA sequences";
RL   ISO/TS 21569-4:2016  (2016).
RX   ISO=69339
XX
RN   [3]
RP   1-94
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2014).
RX   PCR=QL-ELE-00-008.pdf
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..94
FT                   /standard_name="PCR 94 bp amplicon"
FT                   /note="element-specific RT-PCR"
FT                   /target="Nopaline synthase promoter (P-nos) from Agrobacterium tumefaciens"
FT   primer_bind     1..24
FT                   /standard_name="Primer reverse: p-nos-R"  
FT                   /note="TCAGTGGAGCATTTTTGACAAGAA"
FT                   /target="P-nos"
FT   primer_bind     complement(42..67)
FT                   /standard_name="RT-PCR probe: p-nos-Tm"
FT                   /note="FAM-CGCGTTCAAAAGTCGCCTAAGGTCAC-BBQ"
FT   primer_bind     complement(69..94)
FT                   /standard_name="Primer forward: p-nos-F1" 
FT                   /note="AAGCACATACGTCAGAAACCATTATT
FT                   /target="P-nos"
XX
SQ   Sequence 94 BP; 24 A; 15 C; 23 G; 32 T; 0 other;
     tcagtggagc atttttgaca agaannnnnn nnnnnnnnnn ngtgacctta ggcgactttt        60
     gaacgcgnaa taatggtttc tgacgtatgt gctt                                    94
//