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GMOMETHODS:
EU Database of Reference Methods for GMO Analysis
Search for Select by GMO Unique Identifier:
Entry information
Entry name QL-ELE-00-003;       See in JRC GMO-Matrix       See in JRC GMO-Amplicons
GMO Unique Identifier
Description
Description Qualitative PCR method for detection of Neomycin phosphotransferase II gene (BVL L 00.00-31, 1998)
Keywords element_specific.
1 Collection of Official Methods under Article 35 of the German Federal Foods Act; "Screening procedure for the detection of genetically modified DNA sequences in foods by identification of DNA sequences that frequently occur in genetically modified organisms" Food Analysis, L 00.00-31, Beuth, Berlin Koln (1998)
Reference Position 1-173
2 "PCR reactions set up and amplification conditions" Online Publication (2010)
PCR QL-ELE-00-003.pdf
Reference Position 1-173
Features
Key Location Qualifier Value
STS 1..173 standard_namePCR 173 bp amplicon
noteelement-specific
targetNeomycin phosphotransferase II (nptII) gene
primer_b 1..16 standard_namePrimer forward: TN5-1
noteGGATCTCCTGTCATCT
targetnptII
primer_b complement(158..173) standard_namePrimer reverse: TN5-2
noteGATCATCCTGATCGAC
targetnptII
Sequence information
Length: 173 BP, A Count: 6, C Count: 8, T Count: 10, G Count: 8
ggatctcctg tcatctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       120
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtc gatcaggatg atc              173