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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)


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ID   QL-ELE-00-003; SV 0; linear; genomic DNA; STS; SYN; 173 BP.
XX
AC   ;
XX
DT   23-JUN-2009
DT   11-JAN-2018
XX
DE   Qualitative PCR method for detection of Neomycin phosphotransferase II gene
DE   (BVL L 00.00-31, 1998)
XX
KW   element_specific.
XX
RN   [1]
RP   1-173
RA   Collection of Official Methods under Article 35 of the German Federal Foods Act;
RT   "Screening procedure for the detection of genetically modified DNA
RT   sequences in foods by identification of DNA sequences that frequently
RT   occur in genetically modified organisms";
RL   Food Analysis, L 00.00-31, Beuth, Berlin Koln (1998).
XX
RN   [2]
RP   1-173
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QL-ELE-00-003.pdf
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FH   Key             Location/Qualifiers
FH
FT   STS             1..173
FT                   /standard_name="PCR 173 bp amplicon"
FT                   /note="element-specific"
FT                   /target="Neomycin phosphotransferase II (nptII) gene"
FT   primer_bind     1..16
FT                   /standard_name="Primer forward: TN5-1"
FT                   /note="GGATCTCCTGTCATCT"
FT                   /target="nptII"
FT   primer_bind     complement(158..173)
FT                   /standard_name="Primer reverse: TN5-2"
FT                   /note="GATCATCCTGATCGAC"
FT                   /target="nptII"
XX
SQ   Sequence 173 BP; 6 A; 8 C; 8 G; 10 T; 141 other;
     ggatctcctg tcatctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       120
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtc gatcaggatg atc              173
//