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ID   QT-TAX-GM-002; SV 0; linear; genomic DNA; STS; PLN; 74 BP.
XX
AC   ;
XX
DT   29-MAY-2009
DT   24-AUG-2016
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DE   Quantitative PCR method for detection of soybean lectin gene 
XX
KW   taxon_specific, validated_in_combination.
XX
OS   Glycine max (soybean)
XX
RN   [1]
RP   1-74
RT   "Foodstuffs - Methods of analysis for the detection of genetically
RT   modified organisms and derived products - Quantitative nucleic acid based
RT   methods";
RL   ISO 21570:1-103 (2005).
RX   ISO=34615
XX
RN   [2]
RP   1-74
RA   Mazzara M., Munaro B., Larcher S., Grazioli E., Charles Delobel C., Savini C., Van Den Eede G.;
RT   "Event-specific Method for the Quantification of Soybean Line 40-3-2
RT   Using Real-time PCR - Validation Report and Protocol - Report on the
RT   Validation of a DNA Extraction Method for Soybean Seeds.
RL   Online Publication (2007).
RX   DOI=10.2788/61824
XX
RN   [3]
RP   1-74
RA   Charles Delobel C., Bogni A., Pinski G., Mazzara M., Van Den Eede G.;
RT   "Event-specific Method for the Quantification of Soybean Line MON89788 - Validation Report and Protocol";
RL   Online Publication (2008).
RX   DOI=10.2788/44579
XX
RN   [4]
RP   1-74
RA   Mazzara M., Munaro B., Grazioli E., Savini C., Charles Delobel C., Van Den Eede G.;
RT   "Event-specific Method for the Quantification of Soybean Event DP-356043-5 Using Real-time PCR - Validation Report and Protocol";
RL   Online Publication (2009).
RX   DOI=10.2788/5751
XX
RN   [5]
RP   1-74
RA   Mazzara M., Munaro B., Grazioli E., Savini C., Charles Delobel C., Van Den Eede G.;
RT   "Event-specific Method for the Quantification of Soybean Event DP-305423-1 Using Real-time PCR - Validation Report and Protocol";
RL   Online Publication (2009).
RX   DOI=10.2788/57423
XX
RN   [6]
RP   1-74
RA   Delobel C., Mazzara M., Grazioli E., Van den Eede G.;
RT   "Event-specific Method for the Quantification of Soybean MON87701 by Real-time PCR - Validation Report and Validated Method";
RL   Online Publication (2011).
RX   DOI=10.2788/38101
XX
RN   [7]
RP   1-74
RA   Savini C., Mazzara M., Pinski G., Van den Eede G.;
RT   "Event-specific Method for the Quantification of Soybean  CV127 by Real-time PCR - Validation Report and Validated Method";
RL   Online Publication (2011).
RX   DOI=10.2788/37626
XX
RN   [8]
RP   1-74
RA   Mazzara M., Delobel C., Pinski G., Savini C., Van den Eede G.;
RT   "Event-specific Method for the Quantification of Soybean MON87769 Using Real-time PCR - Validation Report and Validated Method";
RL   Online Publication (2012).
RX   DOI=10.2788/4544
XX
RN   [9]
RP   1-74
RA   Savini C.,Mazzara M., Delobel C., Pinski G., Van den Eede G.;
RT   "Event-specific Method for the Quantification of Soybean MON87705 Using Real-time PCR - Validation Report and Validated Method
RL   Online Publication (2012).
RX   DOI=10.2788/48383
XX
RN   [10]
RP   1-74
RA   Savini C., Mazzara M., Munaro B., Kreysa J.;
RT   "Event-specific Method for the Quantification of Soybean MON87708 Using Real-time PCR - Validation Report and Validated Method";
RL   Online Publication (2013).
RX   DOI=10.2788/90943
XX
RN   [11]
RP   1-74
RA   Savini C., Sacco M.G., Mazzara M., Kreysa J.;
RT   "Event-specific Method for the Quantification of Soybean DAS-68416-4 Using Real-time PCR. Validation Report and Validated Method";
RL   Online Publication (2014).
RX   DOI=10.2788/87396
XX
RN   [12]
RP   1-74
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Soybean DAS-81419-2 by Real-time PCR - Validation Report and Validated Method";
RL   Online Publication (2015).
RX   EURL_GMFF=EURLVL0313VR%20Soybean%20DAS-81419-2%20.pdf
RX   EURL_GMFF=EURLVL0313VP_Validated%20method.pdf
XX
RN   [13]
RP   1-74
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Soybean DAS-44406-6 by Real-time PCR - Validation Report and Validated Method";
RL   Online Publication (2015).
RX   EURL_GMFF=EURL-VL-0112%20VR_Final.pdf
RX   EURL_GMFF=EURL-VL-01-12_VP_Final.pdf
XX
RN   [14]
RP   1-74
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Soybean MON 87751 Using Real-time PCR - Validation Report and Validated Method";
RL   Online Publication (2016).
RX   EURL_GMFF=EURL-VL-03-14-VR.pdf
RX   EURL_GMFF=MON-87751-Validated-Method.pdf
XX
RN   [15]
RP   1-74
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Soybean SYHT0H2 by Real-time PCR - JRC Validated Methods, Reference Methods and Measurements Reports";
RL   Online Publication (2016).
RX   EURL_GMFF=EURL-VL-04-12-VR.pdf
RX   EURL_GMFF=EURL-VL-04-12-VP.pdf
XX
RN   [16]
RP   1-74
RT   "See Cross-references below";
RL   Online Publication (2010).
XX
DR   GMOMETHODS; QT-CON-00-003;
DR   GMOMETHODS; QT-EVE-GM-005;
DR   GMOMETHODS; QT-EVE-GM-006;
DR   GMOMETHODS; QT-EVE-GM-009;
DR   GMOMETHODS; QT-EVE-GM-008;
DR   GMOMETHODS; QT-EVE-GM-010;
DR   GMOMETHODS; QT-EVE-GM-011;
DR   GMOMETHODS; QT-EVE-GM-002;
DR   GMOMETHODS; QT-EVE-GM-003;
DR   GMOMETHODS; QT-EVE-GM-012;
DR   GMOMETHODS; QT-EVE-GM-013;
DR   GMOMETHODS; QT-EVE-GM-014;
DR   GMOMETHODS; QT-EVE-GM-015;
DR   GMOMETHODS; QT-EVE-GM-016;
DR   GMOMETHODS; QT-EVE-GM-017;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..74
FT                   /standard_name="PCR 74 bp amplicon"
FT                   /note="taxon-specific RT-PCR for soybean"
FT                   /target="lectin (Le1) gene"
FT   primer_bind     1..20
FT                   /standard_name="Primer forward: GM1-F"
FT                   /note="CCAGCTTCGCCGCTTCCTTC"
FT                   /target="Le1"
FT   primer_bind     23..46
FT                   /standard_name="RT-PCR probe: GM1"
FT                   /note="FAM-CTTCACCTTCTATGCCCCTGACAC-TAMRA"
FT   primer_bind     complement(52..74)
FT                   /standard_name="Primer reverse: GM1-R"
FT                   /note="GAAGGCAAGCCCATCTGCAAGCC"
FT                   /target="Le1"
XX
SQ   Sequence 74 BP; 14 A; 27 C; 13 G; 20 T; 0 other;
     ccagcttcgc cgcttccttc nncttcacct tctatgcccc tgacacnnnn nggcttgcag        60
     atgggcttgc cttc                                                          74
//