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ID   QT-TAX-BN-001; SV 0; linear; genomic DNA; STS; PLN; 129 BP.
XX
AC   ;
XX
DT   03-JUL-2012
DT   05-OCT-2022
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DE   Quantitative PCR method for detection of oilseed rape acyl-ACP thioesterase gene (Jacchia et al., 2014)
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KW   taxon_specific, validated_in_combination.
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OS   Brassica napus (oilseed rape)
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RN   [1]
RP   1-129
RA   Jacchia S., Bogni A., Mazzara M., Kreysa J.;
RT   "Event-specific Method for the Quantification of Oilseed Rape DP-073496-4 Using Real-time PCR - Validation Report and Protocol - Sampling and DNA Extraction from Oilseed Rape";
RL   Online Publication (2014).
RX   EURL_GMFF=EURL-VL-02-12VR-EFSA-Corr1.pdf
RX   EURL_GMFF=EURL-VL-02-12VP-EFSA-Corr1.pdf
XX
RN   [2]
RP   1-129
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Oilseed Rape LBFLFK
RT   Locus1 and Locus2 Using Real-time PCR - Validation Report";
RL   Online Publication (2022).
RX   EURL_GMFF=EURL-VL-02-19-VR.pdf
RX   EURL_GMFF=EURL-VL-02-19-VM.pdf
XX
RN   [3]
RP   1-129
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Oilseed Rape MON 94100
RT   Using Real-time PCR - Validation Report";
RL   Online Publication (2022).
RX   EURL_GMFF=EURL-VL-04-20-VR.pdf
RX   EURL_GMFF=EURL-VL-04-20-VM.pdf
XX
RN   [4]
RP   1-129
RT   "See Cross-references below";
RL   Online Publication (2014).
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DR   GMOMETHODS; QT-EVE-BN-009;
DR   GMOMETHODS; QT-EVE-BN-012;
DR   GMOMETHODS; QT-EVE-BN-013;
DR   GMOMETHODS; QT-EVE-BN-014;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..129
FT                   /standard_name="PCR 126 bp amplicon"
FT                   /note="taxon-specific RT-PCR for oilseed rape"
FT                   /note="A novel A-genome specific oilseed rape PCR detection
FT                   method for acyl-ACP thioesterase (FatA) gene in Brassica
FT                   napus, Brassica rapa and Brassica juncea. The amplified FatA(A) fragment is 126 bp (missing the NNN nucleotides in the 129 bp amplicon below) in a majority of Brassica napus varieties, in all Brassica juncea varieties and in some of the Brassica rapa varieties tested; it is of 129 bp in a minority of Brassica napus varieties and in some Brassica rapa varieties tested.;
FT                   /target="acyl-ACP thioesterase (FatA) gene"
FT   primer_bind     1..24
FT                   /standard_name="Primer forward: 09-0-3249"
FT                   /note="ACAGATGAAGTTCGGGACGAGTAC"
FT                   /target="FatA"
FT   primer_bind     54..73
FT                   /standard_name="RT-PCR probe: 09-QP-87"
FT                   /note="FAM-AAGAAGAATCATCATGCTTC-MGBNFQ"
FT   N_region        77..79
FT                   /note="'N' bases represent oligonucleotide sequences generally missing 
FT                   in B. napus, B. rapa and B.
FT                   juncea."
FT   primer_bind     complement(103..129)
FT                   /standard_name="Primer reverse: 09-0-3251"
FT                   /note="CAGGTTGAGATCCACATGCTTAAATAT"
FT                   /target="FatA"
XX
SQ   Sequence 129 BP; 37 A; 22 C; 27 G; 38 T; 5 other;
     acagatgaag ttcgggacga gtacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaagaaga        60
     atcatcatgc ttcnnnNNNn nnnnnnnnnn nnnnnnnnnn nnatatttaa gcatgtggat       120
     ctcaacctg                                                               129
//