ID   QT-EVE-GM-019; SV 0; linear; genomic DNA; STD; PLN; 89 BP.
XX
AC   DBN-09004-6;
XX
DT   01-JAN-2019
DT   07-NOV-2023
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DE   Quantitative PCR method for detection of soybean event DBN-09004-6 (EURL GMFF, 2023).
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KW   event_specific.
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OS   Glycine max (soybean) - event DBN-09004-6 (DBN-09004-6)
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RN   [1]
RP   1-89
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Soybean DBN-09004-6
RT   Using Real-time PCR - Validation Report";
RL   Online Publication (2023).
RX   EURL_GMFF=EURL-VL-08-19-VR.pdf
RX   EURL_GMFF=EURL-VL-08-19-VM.pdf
XX
RN   [2]
RP   1-89
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2023).
RX   PCR=QT-EVE-GM-019.pdf
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DR   GMOMETHODS; QT-TAX-GM-005;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..89
FT                   /standard_name="PCR 89 bp amplicon"
FT                   /note="Event-specific RT-PCR"
FT                   /target="3' integration border region (IBR) between the insert of soybean event DBN-09004-6 and the soybean host genome"
FT   primer_bind     1..20
FT                   /standard_name="Primer forward: S17A01_F"
FT                   /note="GCCGTATCCGCAATGTGTTA"
FT                   /target="insert"
FT   primer_bind     42..62
FT                   /standard_name="RT-PCR probe: S17A01_P"
FT                   /note="FAM-TTGTTTACAACTCTGTGACCC-MGBNFQ"
FT   primer_bind     complement(68..89)
FT                   /standard_name="Primer reverse: S17A01_R"
FT                   /note="GCTCCATAAACGTGTGCTTTCA"
FT                   /target="3'-host genome"
XX
SQ   Sequence 89 BP; 23 A; 19 C; 19 G; 28 T; 0 other;
     gccgtatccg caatgtgtta nnnnnnnnnn nnnnnnnnnn nttgtttaca actctgtgac        60
     ccnnnnntga aagcacacgt ttatggagc                                          89
//