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ID   QT-EVE-GM-005; SV 0; linear; genomic DNA; STS; PLN; 84 BP.
XX
AC   MON-04032-6;
XX
DT   18-APR-2005
DT   12-OCT-2010
XX
DE   Quantitative PCR method for detection of soybean event GTS-40-3-2
DE   (Mazzara et al., 2007).
XX
KW   event_specific.
XX
OS   Glycine max (soybean) - event GTS-40-3-2 (MON-04032-6)
XX
RN   [1]
RP   1-84
RA   European Commission, Joint Research Centre; 
RT   "Event-specific Method for the Quantification of Soybean Line 40-3-2
RT   Using Real-time PCR - Validation Report and Protocol - Report on the
RT   Validation of a DNA Extraction Method for Soybean Seeds."
RL   Online Publication (2018).
RX   DOI=10.2760/720444
XX
RN   [2]
RP   1-84
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QT-EVE-GM-005.pdf
XX
DR   GMOMETHODS; QT-TAX-GM-002;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..84
FT                   /standard_name="PCR 84 bp amplicon"
FT                   /note="event-specific RT-PCR"
FT                   /target="5' integration border region (IBR) between the insert of soybean event GTS 40-3-2 and the soybean host genome"
FT   primer_bind     1..31
FT                   /standard_name="Primer forward: 40-3-2 AF"
FT                   /note="TTCATTCAAAATAAGATCATACATACAGGTT"
FT                   /target="5'-host genome"
FT   primer_bind     complement(49..63)
FT                   /standard_name="RT-PCR probe: 40-3-2 AP"
FT                   /note="FAM-CCTTTTCCATTTGGG-MGBNFQ"
FT   primer_bind     complement(64..84)
FT                   /standard_name="Primer reverse: 40-3-2 AR"
FT                   /note="GGCATTTGTAGGAGCCACCTT"
FT                   /target="insert"
XX
SQ   Sequence 84 BP; 26 A; 14 C; 12 G; 15 T; 17 other;
     ttcattcaaa ataagatcat acatacaggt tnnnnnnnnn nnnnnnnncc caaatggaaa        60
     aggaaggtgg ctcctacaaa tgcc                                               84
//