Perform your search by keyword, select a GMO unique identifier or click a link in the section below.


ID   QT-EVE-GH-011; SV 0; linear; genomic DNA; STD; SYN; 120 BP.
XX
AC   DAS-81910-7;
XX
DT   07-OCT-2016
DT   10-DEC-2019
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DE   Quantitative PCR method for detection of cotton event DAS-81910-7 (EURL GMFF, 2019).
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KW    event_specific.
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OS   Gossypium hirsutum (upland cotton) - event DAS-81910-7 (DAS-81910-7)
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RN   [1]
RP   1-120
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Cotton DAS-81910-7 Using Real-time PCR - Validation Report";
RL   Online Publication (2019).
RX   EURL_GMFF=EURL-VL-06_16_VR.pdf
RX   EURL_GMFF=EURL-VL-06_16_VP.pdf
XX
RN   [2]
RP   1-120
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2019).
RX   PCR=QT-EVE-GH-011.pdf
XX
DR   GMOMETHODS; QT-TAX-GH-021;
DR   GMOMETHODS; QT-TAX-GH-016;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..120
FT                   /standard_name="PCR 120 bp amplicon"
FT                   /note="Event-specific RT-PCR";
FT                   /target="5' integration border region (IBR) between the insert of cotton event DAS-81910-7 and the cotton host genome"
FT   primer_bind     1..23
FT                   /standard_name="Primer forward: 1706-f2"
FT                   /note="AAGCTTAGGTGATTTCGATGATG"
FT                   /target="5'-host genome"
FT   primer_bind     66..85
FT                   /standard_name="RT-PCR probe: 1706-p3"
FT                   /note="FAM-CACACCAAAAGTTAGGCCCG-TAMRA"
FT   primer_bind     complement(101..120)
FT                   /standard_name="Primer reverse: 1706-r3"
FT                   /note="GACCTCAATTGCGAGCTTTC"
FT                   /target="insert"
XX
SQ   Sequence 120 BP; 41 A; 19 C; 24 G; 36 T; 0 other;
     aagcttaggt gatttcgatg atgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnnnncacac caaaagttag gcccgnnnnn nnnnnnnnnn gaaagctcgc aattgaggtc       120
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