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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)


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ID   QL-TAX-ZM-003; SV 0; linear; genomic DNA; STS; PLN; 225 BP.
XX
AC   ;
XX
DT   23-JUN-2009
DT   23-SEP-2010
XX
DE   Qualitative PCR method for detection of maize invertase gene
DE   (ISO/FDIS 21569:2005).
XX
KW   taxon_specific, validated_independently.
XX
OS   Zea mays (maize)
XX
RN   [1]
RP   1-225
RT   "Foodstuffs - Methods of analysis for the detection of genetically
RT   modified organisms and derived products - Qualitative nucleic acid based
RT   methods";
RL   ISO 21569:1-69 (2005).
RX   ISO=34614
XX
RN   [2]
RP   1-225
RT   "Detection of a genetic modification of maize (Zea mays L.) by
RT   amplification of the modified DNA sequence by means of the polymerase
RT   chain reaction (PCR) and hybridization of the PCR product with a DNA
RT   probe or restriction analysis of the PCR product, N. L 15.05-1";
RL   Online Publication (2002).
XX
RN   [3]
RP   1-225
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QL-TAX-ZM-003.pdf
XX
DR   GMOMETHODS; QL-CON-00-003;
DR   GMOMETHODS; QL-CON-00-004;
DR   GMOMETHODS; QL-EVE-ZM-001;
DR   GMOMETHODS; QL-CON-00-005;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..225
FT                   /standard_name="PCR 225 bp amplicon"
FT                   /note="Taxon-specific PCR for maize"
FT                   /target="invertase (Ivr1) gene"
FT   primer_bind     1..25
FT                   /standard_name="Primer forward: IVR1-F"
FT                   /note="CCGCTGTATCACAAGGGCTGGTACC"
FT                   /target="Ivr1"
FT   primer_bind     complement(201..225)
FT                   /standard_name="Primer reverse: IVR1-R"
FT                   /note="GGAGCCCGTGTAGAGCATGACGATC"
FT                   /target="Ivr1"
XX
SQ   Sequence 225 BP; 34 A; 88 C; 65 G; 38 T; 0 other;
     ccgctgtatc acaagggctg gtaccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       120
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       180
     nnnnnnnnnn nnnnnnnnnn gatcgtcatg ctctacacgg gctcc                       225
//