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GMOMETHODS:
EU Database of Reference Methods for GMO Analysis
Search for Select by GMO Unique Identifier:
Entry information
Entry name QL-TAX-GM-008;  
GMO Unique Identifier
Description
Description Qualitative PCR method for detection of soybean lectin gene (EU-Project SMT4-CT96-2072:1998).
Keywords taxon_specific, validated_in_combination.
From Glycine max (soybean)
References
1 "Developments of Methods to Identify Foods Produced by Means of Genetic Engineering Techniques (DMIF-GEN) - Final Report" EU-Project SMT4-CT96-2072:1-99 (1998)
Reference Position 1-118
2 "See Cross-references below" Online Publication (2010)
Reference Position 1-118
Cross-references
GMOMETHODS QL-CON-00-006;
Features
Key Location Qualifier Value
STS 1..118 standard_namePCR 118 bp amplicon
noteTaxon-specific PCR for soybean
targetlectin (Le1) gene
primer_b 1..22 standard_namePrimer forward: GM03
noteGCCCTCTACTCCACCCCCATCC
targetLe1
primer_b complement(96..118) standard_namePrimer reverse: GM04
noteGCCCATCTGCAAGCCTTTTTGTG
targetLe1
Sequence information
Length: 118 BP, A Count: 27, C Count: 43, T Count: 26, G Count: 22
gccctctact ccacccccat ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncacaa aaaggcttgc agatgggc         118