ID   QL-TAX-GM-002; SV 0; linear; genomic DNA; STS; PLN; 118 BP.
XX
AC   ;
XX
DT   23-JUN-2009
DT   01-OCT-2010
XX
DE   Qualitative PCR method for detection of soybean lectin gene
DE   (ISO/FDIS 21569:2005).
XX
KW   taxon_specific, validated_independently.
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OS   Glycine max (soybean)
XX
RN   [1]
RP   1-118
RT   "Foodstuffs - Methods of analysis for the detection of genetically
RT   modified organisms and derived products - Qualitative nucleic acid based
RT   methods";
RL   ISO 21569:1-69 (2005).
RX   ISO=34614
XX
RN   [2]
RP   1-118
RT   "Detection of a genetic modification of soybeans by amplification of the
RT   modified DNA sequence by means of the polymerase chain reaction (PCR) and
RT   hybridization of the PCR product with a DNA probe, N. L23.01.22-1";
RL   Online Publication (1998).
XX
RN   [3]
RP   1-118
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QL-TAX-GM-002.pdf
XX
DR   GMOMETHODS; QL-CON-00-001;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..118
FT                   /standard_name="PCR 118 bp amplicon"
FT                   /note="Taxon-specific PCR for soybean"
FT                   /target="lectin (Le1) gene"
FT   primer_bind     1..22
FT                   /standard_name="Primer forward: GM03"
FT                   /note="GCCCTCTACTCCACCCCCATCC"
FT                   /target="Le1"
FT   primer_bind     complement(96..118)
FT                   /standard_name="Primer reverse: GM04"
FT                   /note="GCCCATCTGCAAGCCTTTTTGTG"
FT                   /target="Le1"
XX
SQ   Sequence 118 BP; 27 A; 43 C; 22 G; 26 T; 0 other;
     gccctctact ccacccccat ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncacaa aaaggcttgc agatgggc         118
//