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ID   QL-TAX-CMV-001; SV 1; circular; genomic DNA; STD; VRL; 68 BP.
XX
AC   ;
XX
DT   14-NOV-2023
DT   14-NOV-2023
XX
DE   Qualitative PCR method for detection of CaMV ORF V (ISO/TS 21569-7, 2022).
XX
KW   taxon_specific, validated_independently.
XX
OS   Cauliflower mosaic virus
XX
RN   [1]
RP   1-68
RT   "Horizontal methods for molecular biomarker analysis - Methods of
RT   analysis for the detection of genetically modified organisms and derived
RT   products - Part 7: Real-time PCR based methods for the detection of CaMV
RT   and Agrobacterium Ti-plasmid derived DNA sequences";
RL   ISO/TS 21569-7 (2022).
RX   ISO=84434
XX
RN   [2]
RP   1-68
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2023).
RX   PCR=QL-TAX-CMV-001.pdf
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..68
FT                   /standard_name="PCR 68 bp amplicon"
FT                   /note="Taxon-specific RT-PCR"
FT                   /target="Cauliflower mosaic virus (CaMV) open reading frame V (ORF V)"
FT   primer_bind     1..20
FT                   /standard_name="Primer forward: CaMV-ORFV-fd2"
FT                   /note="ATYAAGCCCAGYAAAAGCCC"
FT                   /target="CaMV ORF V"
FT   primer_bind     24..48
FT                   /standard_name="RT-PCR probe: CaMV-ORFV-P2"
FT                   /note="FAM-CATGGCACCAGCCTTCTTGGTCAAC-BHQ1"
FT   primer_bind     complement(49..68)
FT                   /standard_name="Primer reverse: CaMV-ORFV-rd2"
FT                   /note="CTYCGCTTCTCGGCTTCRTT"
FT                   /target="CaMV ORF V"
XX
SQ   Sequence 68 BP; 23 A; 19 C; 15 G; 11 T; 0 other;
     atyaagccca gyaaaagccc nnncatggca ccagccttct tggtcaacaa ygaagccgag        60
     aagcgrag                                                                 68
//