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EU Database of Reference Methods for GMO Analysis
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Entry information
Entry name QL-ELE-00-016;       See in JRC GMO-Matrix       See in JRC GMO-Amplicons
GMO Unique Identifier
Description Qualitative PCR method for detection of cry1Ab/Ac gene
Keywords element_specific.
1 Grohmann L., Reiting R., Maede D., Uhlig S., Simon K., Frost K., Jit Randhawa G., Zur K.; "Collaborative trial validation of cry1Ab/Ac and Pubi-cry TaqMan-based real-time PCR assays for detection of DNA derived from genetically modified Bt plant products" Accred Qual Assur 20:85-96 (2015)
DOI 10.1007/s00769-015-1108-5
Reference Position 1-74
2 "Detection of genetically modified cry1Ab/Ac and P-ubi-cry DNA sequences in rice products using real-time PCR" BVL L 15.06-3:2013-08 (2013)
BVL bvl-l-1506-3/193413251
Reference Position 1-74
3 "Horizontal methods for molecular biomarker analysis -- Methods of analysis for the detection of genetically modified organisms and derived products -- Part 6: Real-time PCR based screening methods for the detection of cry1Ab/Ac and Pubi-cry DNA sequences" ISO/TS 21569-6:2016 (2016)
ISO 69356
Reference Position 1-74
4 "PCR reactions set up and amplification conditions" Online Publication (2014)
PCR QL-ELE-00-016.pdf
Reference Position 1-74
Key Location Qualifier Value
STS 1..74 standard_namePCR 74 bp amplicon
noteelement-specific RT-PCR
targetcry1Ab/Ac synthetic construct derived from Bacillus thuringiensis
primer_b 1..24 standard_namePrimer forward: Bt-F1
primer_b 26..50 standard_nameRT-PCR probe: Bt-P
primer_b complement(55..74) standard_namePrimer reverse: Bt-R
Sequence information
Length: 74 BP, A Count: 23, C Count: 20, T Count: 16, G Count: 15
gaggaaatgc gtattcaatt caacnacatg aacagcgcct tgaccacagc nnnnccattg        60
ttcgcagtcc agaa                                                          74