EU Database of Reference Methods for GMO Analysis - Joint Research Centre (JRC), European Commission

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Entry information

Entry name

QL-CON-00-006; See in JRC GMO-Matrix See in JRC GMO-Amplicons

GMO Unique Identifier

MON-04032-6

Description

Qualitative PCR method for detection of the junction between the CaMV35S promoter and the chloroplast transit peptide sequence (EU-Project SMT4-CT96-2072:1998).

Keywords

construct_specific.

From

Glycine max (soybean) - event GTS-40-3-2 (MON-04032-6)

References

"Developments of Methods to Identify Foods Produced by Means of Genetic Engineering Techniques (DMIF-GEN) - Final Report" EU-Project SMT4-CT96-2072:1-99 (1998)

Reference Position

1-172

"PCR reactions set up and amplification conditions" Online Publication (2010)

PCR	QL-CON-00-006.pdf
Reference Position	1-172

Cross-references

GMOMETHODS

QL-TAX-GM-008;

Features

Key	Location	Qualifier	Value
STS	1..172	standard_name	PCR 172 bp amplicon
		note	Construct-specific PCR
		target	Junction region between the Cauliflower Mosaic Virus 35S promoter (CaMV P-35S) and the chloroplast-transit-peptide (CTP) sequence from Petunia hybrida epsps gene
primer_b	1..22	standard_name	Primer forward: p35S-af2
		note	TGATGTGATATCTCCACTGACG
		target	CaMV P-35S
primer_b	complement(151..172)	standard_name	Primer reverse: petu-ar1
		note	TGTATCCCTTGAGCCATGTTGT
		target	CTP4

Sequence information

Length: 172 BP, A Count: 14, C Count: 10, T Count: 10, G Count: 10

tgatgtgata tctccactga cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       120
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acaacatggc tcaagggata ca               172