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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)


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ID   QL-CON-00-005; SV 0; linear; genomic DNA; STS; SYN; 209 BP.
XX
AC   ACS-ZM003-2;
XX
DT   23-JUN-2009
DT   05-OCT-2016
XX
DE   Qualitative PCR method for detection of the junction between the pat gene and the CaMV35S terminator (ISO/FDIS 21569:2005).
XX
KW   construct_specific.
XX
OS   Zea mays (maize) - event T25 (ACS-ZM003-2)
XX
RN   [1]
RP   1-209
RT   "Foodstuffs - Methods of analysis for the detection of genetically
RT   modified organisms and derived products - Qualitative nucleic acid based
RT   methods";
RL   ISO 21569:1-69 (2005).
RX   ISO=34614
XX
RN   [2]
RP   1-209
RT   "Detection of a genetic modification of maize (Zea mays L.) by
RT   amplification of the modified DNA sequence by means of the polymerase
RT   chain reaction (PCR) and hybridization of the PCR product with a DNA
RT   probe or restriction analysis of the PCR product, N. L 15.05-1";
RL   Online Publication (2002).
XX
RN   [3]
RP   1-209
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QL-CON-00-005.pdf
XX
DR   GMOMETHODS; QL-TAX-ZM-003;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..209
FT                   /standard_name="PCR 209 bp amplicon"
FT                   /note="Construct-specific PCR"
FT                   /target="Junction region between the phosphinothricin N-acetyltransferase (pat) gene from Streptomyces viridochromogenes and the Cauliflower Mosaic Virus 35S terminator (CaMV T-35S)"
FT   primer_bind     1..21
FT                   /standard_name="Primer forward: T25-F7"
FT                   /note="ATGGTGGATGGCATGATGTTG"
FT                   /target="pat"
FT   primer_bind     complement(187..209)
FT                   /standard_name="Primer reverse: T25-R3"
FT                   /note="TGAGCGAAACCCTATAAGAACCC"
FT                   /target="CaMV T-35S"
XX
SQ   Sequence 209 BP; 7 A; 5 C; 16 G; 16 T; 165 other;
     atggtggatg gcatgatgtt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       120
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       180
     nnnnnngggt tcttataggg tttcgctca                                         209
//