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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)


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ID   QL-CON-00-003; SV 0; linear; genomic DNA; STS; SYN; 189 BP.
XX
AC   SYN-BT011-1;
XX
DT   23-JUN-2009
DT   05-OCT-2016
XX
DE   Qualitative PCR method for detection of the junction between the intron 2 from the maize adh1 gene and the pat gene(ISO/FDIS 21569:2005).
XX
KW   construct_specific.
XX
OS   Zea mays (maize) - event Bt11 (SYN-BT011-1)
XX
RN   [1]
RP   1-189
RT   "Foodstuffs - Methods of analysis for the detection of genetically
RT   modified organisms and derived products - Qualitative nucleic acid based
RT   methods";
RL   ISO 21569:1-69 (2005).
RX   ISO=34614
XX
RN   [2]
RP   1-189
RT   "Detection of a genetic modification of maize (Zea mays L.) by
RT   amplification of the modified DNA sequence by means of the polymerase
RT   chain reaction (PCR) and hybridization of the PCR product with a DNA
RT   probe or restriction analysis of the PCR product, N. L 15.05-1";
RL   Online Publication (2002).
XX
RN   [3]
RP   1-189
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QL-CON-00-003.pdf
XX
DR   GMOMETHODS; QL-TAX-ZM-003;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..189
FT                   /standard_name="PCR 189 bp amplicon"
FT                   /note="Construct-specific PCR"
FT                   /target="Junction region between the Intron 2 (IVS2) from the maize alcohol dehydrogenase 1 (adh1) gene and the phosphinothricin N-acetyltransferase (pat) gene"
FT   primer_bind     1..22
FT                   /standard_name="Primer forward: IVS2-2"
FT                   /note="CTGGGAGGCCAAGGTATCTAAT"
FT                   /target="IVS 2 adh1"
FT   primer_bind     complement(168..189)
FT                   /standard_name="Primer reverse: PAT-B"
FT                   /note="GCTGCTGTAGCTGGCCTAATCT"
FT                   /target="pat"
XX
SQ   Sequence 189 BP; 40 A; 50 C; 50 G; 49 T; 0 other;
     ctgggaggcc aaggtatcta atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       120
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaga ttaggccagc       180
     tacagcagc                                                               189
//