Match details
| Record ID: |
JQ309940.2 |
| Database: |
nt |
Go to original record |
| Description: |
Brassica napus clone MS1L left border of T-DNA genomic sequence. |
| Taxon: |
Brassica napus |
| Original size: |
1103 bp |
View other 1 match for this record
| Method: |
QT-EVE-BN-005 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
|
| Description: | Quantitative PCR method for detection of oilseed rape event Ms1 (verified by the EU-RL GMFF in the context of Commission Decision 2007/305/EC) |
| Target: | Ms1 |
View other 1 match for this method
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 0 |
0 |
191 |
377 |
+ |
187 bp |
|
1 acgctgcgga catctacatt tttgaattga aaaaaaattg gtaattactc
51 tttctttttc tccatattga ccatcatact cattgctgat ccacctagcc
101 gactttttta aataaccata tcccactatc catcaggcat aaataaccat
151 atcccgtcga tgcttccatc ttcagcttcc gatctag
View other 1 match with an identical amplicon
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
20 bp |
0 |
0 |
1 |
20 |
8.88e-09 |
| primerB |
100.0 |
22 bp |
0 |
0 |
187 |
166 |
7.85e-10 |
| probe |
100.0 |
27 bp |
0 |
0 |
79 |
105 |
1.71e-12 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
