Match details

Record ID: OR392453.1
Database: nt Go to original record
Description: Vector pMON521-ToMoV{A}1.5mer, complete sequence.
Taxon: Vector pMON521-ToMoV{A}1.5mer
Original size: 14747 bp

View other 17 matches for this record


Method: QL-CON-00-010 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of flax event FP967
Target:T-NOS-dhfr

View other 1 match for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 13516 13620 - 105 bp

Amplicon: 105 bp
1     agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
51    gatcgatcaa gtagatacac tacatatatc tacaatagac atcgagccgg
101   aaggt 

View other 1 match with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 5.26e-09
primerB 100.0 20 bp 0 0 105 86 5.26e-09
probe 100.0 20 bp 0 0 25 44 5.26e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.