European Commission logo
Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: OP672271.1
Database: nt Go to original record
Description: Vector PK2-Cas9, complete sequence.
Taxon: Vector PK2-Cas9
Original size: 13797 bp

View other 16 matches for this record

Method: QL-CON-00-014 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the nos promoter and the neomycin phosphotransferase II gene
Target:P-nos-nptII

View other 1572 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 13407 13546 - 140 bp
Amplicon: 140 bp 
1     ttcccctcgg tatccaatta gagtctcata ttcactctca actcgatcga
51    ggcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
101   ggagaggcta ttcggctatg actgggcaca acagacaatc

View other 151 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 1.72e-10
primerB 100.0 22 bp 0 0 140 119 5.81e-10
probe 100.0 25 bp 0 0 117 93 1.48e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.