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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: JX139721.1
Database: nt Go to original record
Description: Brassica napus transgenic strain T45 GM cassette, partial sequence.
Taxon: Brassica napus
Original size: 847 bp

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Method: QT-EVE-BN-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of oilseed rape event T45 (Charles Delobel et al., 2006).
Target:T45

View other 3 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 231 353 - 123 bp
Amplicon: 123 bp 
1     caatggacac atgaattatg catatggaat acagttgtaa atgaattccc
51    atggagtcaa agattcaaat agaggaccta acagaactcg ccgtaaagac
101   tggcgaacag ttcatacaga gtc

View other 3 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 1.84e-09
primerB 100.0 21 bp 0 0 123 103 1.84e-09
probe 100.0 25 bp 0 0 70 94 1.30e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.