Match details

Record ID: JQ406879.1
Database: nt Go to original record
Description: Zea mays transgenic MON810 5' insertion site genomic sequence.
Taxon: Zea mays
Original size: 1018 bp

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Method: QT-EVE-ZM-020 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of maize event MON810.
Target:MON 810

View other 6 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 906 997 + 92 bp

Amplicon: 92 bp
1     tcgaaggacg aaggactcta acgtttaaca tcctttgcca ttgcccagct
51    atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gc 

View other 13 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 24 bp 0 0 1 24 3.46e-11
primerB 100.0 24 bp 0 0 92 69 3.46e-11
probe 100.0 23 bp 0 0 27 49 1.18e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.