Match details

Record ID: AB684632.1
Database: nt Go to original record
Description: Cloning vector pSolycp00007 DNA, complete sequence.
Taxon: Cloning vector pSolycp00007
Original size: 11812 bp

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Method: QL-ELE-00-018 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (Barbau-Piednoir et al., 2014).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 3284 3352 + 69 bp

Amplicon: 69 bp
1     gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
51    agcgcgcaaa ctaggataa 

View other 15381 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 28 bp 0 0 1 28 1.84e-13
primerB 96.0 25 bp 1 0 69 45 9.65e-11


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.