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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: AY592954.1
Database: nt Go to original record
Description: Glycine max transgenic chloroplast CP4-EPSPS fusion protein precursor, gene, promoter region and partial cds; nuclear gene for chloroplast product.
Taxon: Glycine max
Original size: 707 bp

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Method: QT-CON-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of the junction between the CTP sequence and the CP4 epsps gene (Hird et al., 2003).
Target:CTP-CP4 epsps

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 470 557 + 88 bp
Amplicon: 88 bp 
1     ggatttcagc atcagtggct acagcctgca tgcttcacgg tgcaagcagc
51    cggcccgcaa ccgcccgcaa atcctctggc ctttccgg

View other 24 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 1.13e-10
primerB 100.0 18 bp 0 0 88 71 4.89e-08
probe 100.0 21 bp 0 0 53 33 1.29e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.