JRC GMO-Amplicons | EURL GMFF

Record ID:	AY592954.1
Database:	nt	Go to original record
Description:	Glycine max transgenic chloroplast CP4-EPSPS fusion protein precursor, gene, promoter region and partial cds; nuclear gene for chloroplast product.
Taxon:	Glycine max
Original size:	707 bp

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Method:	QL-CON-00-001	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of the junction between the CaMV35S promoter and the chloroplast transit peptide sequence (ISO/FDIS 21569:2005).
Target:	CaMV P-35S-CTP

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
1	0	147	317	+	171 bp

Amplicon:

171 bp

1     tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
51    ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga
101   cacgctgaca agctgactct agcagatctt tcaagaatgg cacaaattaa
151   caacatggca caagggatac a

View other 13 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	22 bp	0	0	1	22	7.16e-10

Match details