European Commission logo
Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: AY562536.1
Database: nt Go to original record
Description: Binary Vector pORE_E3, complete sequence.
Taxon: Binary vector pORE_E3
Original size: 7786 bp

View other 12 matches for this record

Method: QT-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of phosphinothricin N-acetyltransferase gene (Weighard et al., 2004).
Target:pat

View other 1579 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 7417 7484 + 68 bp
Amplicon: 68 bp 
1     ttgagggtgt tgtggctggt attgcttacg ctggaccttg gaaggctagg
51    aacgcttacg attggaca

View other 10 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 9.83e-10
primerB 100.0 21 bp 0 0 68 48 9.83e-10
probe 90.9 22 bp 2 0 44 23 6.28e-07

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.