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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: CP058063.1
Database: nt Go to original record
Description: Escherichia coli strain RHB02-C19 chromosome, complete genome.
Taxon: Escherichia coli
Original size: 5091098 bp
Method: QL-EVE-EC-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of E. coli K-12 event 19E (EURL GMFF, 2015).
Target:E. coli K-12 event 19E
Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 3709812 3709888 + 77 bp
Amplicon: 77 bp 
1     ggttatccag taatagccat cttcatcgcg acgcgcgcct gtattgattc
51    acgagaagta cgaaaaaaac cgggagg
Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 27 bp 0 0 1 27 7.13e-13
primerB 100.0 22 bp 0 0 77 56 3.32e-10
probe 87.5 24 bp 1 2 29 52 1.15e-05

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.