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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: AY553053.1
Database: nt Go to original record
Description: Reporter vector pGSA1370, complete sequence.
Taxon: Reporter vector pGSA1370
Original size: 10695 bp

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Method: QL-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998).
Target:CaMV P-35S

View other 10854 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 3644 3840 - 197 bp
Amplicon: 197 bp 
1     gctcctacaa atgccatcat tgcgataaag gaaaggctat cattcaagat
51    ctgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
101   atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
151   atgtgacatc tccactgacg taagggatga cgcacaatcc cactatc

View other 35 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 3.13e-08
primerB 100.0 20 bp 0 0 197 178 9.38e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.