Match details

Record ID: X83423.1
Database: nt Go to original record
Description: P.capsici serine/threonine and tyrosine kinase genes
Taxon: Phytophthora capsici
Original size: 2979 bp

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Method: QL-ELE-00-011 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator.
Target:T-nos

View other 15584 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 68 151 - 84 bp

Amplicon: 84 bp
1     catgtaatgc atgacgtaat ttatgagatg ggtttttatg attagagtcc
51    cgcaataata catttaatac gcgatagaaa acaa 

View other 1 match with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 96.0 25 bp 1 0 1 25 4.28e-10
primerB 100.0 25 bp 0 0 84 60 9.20e-12
probe 100.0 28 bp 0 0 28 55 2.28e-13


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.