Match details
| Record ID: |
AB180965.1 |
| Database: |
nt |
Go to original record |
| Description: |
Glycine max DNA, transgene of Roundup Ready soybean, clone: 12. |
| Taxon: |
Glycine max |
| Original size: |
473 bp |
View other 1 match for this record
| Method: |
QT-CON-00-003 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
|
| Description: | Quantitative PCR method for detection of the junction between the CaMV35S promoter and the CTP sequence (ISO/FDIS 21570:2005). |
| Target: | CaMV P-35S-CTP |
View other 11 matches for this method
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 1 |
0 |
85 |
158 |
+ |
74 bp |
|
1 catttggaga ggacacgctg acaagccgac tctagcagat ctttcaagaa
51 tggcacaaat taacaacatg gcac
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
21 bp |
0 |
0 |
1 |
21 |
1.08e-09 |
| primerB |
100.0 |
21 bp |
0 |
0 |
72 |
52 |
1.21e-09 |
| probe |
95.8 |
24 bp |
1 |
0 |
22 |
45 |
1.28e-09 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
