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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: AY125353.1
Database: nt Go to original record
Description: Synthetic construct CP4EPSPS protein (CP4EPSPS) gene, complete cds.
Taxon: synthetic construct
Original size: 1946 bp

View other 8 matches for this record

Method: QT-CON-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of the junction between the chloroplast transit peptide and the CP4 epsps gene.
Target:CTP-CP4 epsps

View other 26 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 125 245 + 121 bp
Amplicon: 121 bp 
1     cctttaggat ttcagcatca gtggctacag cctgcatgct tcacggtgca
51    agcagccggc ccgcaaccgc ccgcaaatcc tctggccttt ccggaaccgt
101   ccgcattccc ggcgacaagt c

View other 22 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 24 bp 0 0 1 24 4.34e-11
primerB 100.0 18 bp 0 0 121 104 6.84e-08
probe 100.0 19 bp 0 0 62 80 2.05e-08

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.