Match details

Record ID: AP018645.1
Database: nt Go to original record
Description: Gateway vector R4L1pMpGWB239 DNA, complete sequence.
Taxon: Gateway vector R4L1pMpGWB239
Original size: 11452 bp

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Method: QL-ELE-00-023 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of T35S pCAMBIA sequences (Rischitor et al., 2016).
Target:T35S pCAMBIA

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 3273 3407 + 135 bp

Amplicon: 135 bp
1     cgggggatct ggattttagt actggatttt ggttttagga attagatttt
51    attgatagaa gtattttaca aatacaaata catactaagg gtttcttata
101   tgctcaacac atgagcgaaa ccctatagga accct 

View other 55 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 2.03e-09
primerB 100.0 23 bp 0 0 135 113 1.65e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.