Match details
| Record ID: |
EU090199.1 |
| Database: |
nt |
Go to original record |
| Description: |
Brassica napus trangenic line Rf1 right border junction sequence of transgenic event genomic sequence. |
| Taxon: |
Brassica napus |
| Original size: |
3192 bp |
| Method: |
QT-EVE-BN-006 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
|
| Description: | Quantitative PCR method for detection of oilseed rape event RF1 (verified by the EU-RL GMFF in the context of Commission Decision 2007/305/EC) |
| Target: | RF1 |
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 0 |
0 |
301 |
413 |
- |
113 bp |
|
1 ctaagggagg tcaagatgta gcaggcgaag aacccttttc catcttttcc
51 ggtgactaca cgcgactcat catcctcacc cagtcagcat catcacacca
101 aaagttaggc ccg
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
22 bp |
0 |
0 |
1 |
22 |
4.98e-10 |
| primerB |
100.0 |
20 bp |
0 |
0 |
113 |
94 |
5.68e-09 |
| probe |
100.0 |
27 bp |
0 |
0 |
66 |
92 |
1.01e-12 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
